{"product_id":"proteintech-19262-1-ap","title":"Proteintech, 19262-1-AP, CDC42EP4 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe CDC42EP4 (19262-1-AP) by Proteintech is a Polyclonal antibody targeting CDC42EP4 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n19262-1-AP targets CDC42EP4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: K-562 cells,  A549 cells,  mouse lung tissue\nPositive IHC detected in: human intrahepatic cholangiocarcinoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:3000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCDC42EP4, or Cell division cycle 42 effector protein 4, is a member of the CDC42-binding protein family. This protein interacts with Rho family GTPases and plays a role in regulating the organization of the actin cytoskeleton. It has been shown to bind both CDC42 and TC10 GTPases in a GTP-dependent manner. Overexpression of CDC42EP4 in fibroblasts can induce pseudopodia formation, suggesting its involvement in actin filament assembly and cell shape control. CDC42EP4 is also implicated in cancer progression, particularly in prostate cancer. Furthermore, CDC42EP4 has been implicated in the regulation of glutamate transporters, which are crucial for maintaining glutamate homeostasis in the nervous system. It has been suggested that CDC42EP4 may interact with septins, a family of polymerizing GTPases that constitute the membrane skeleton, and this interaction could potentially influence the localization and activity of glutamate transporters.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag13594 Product name: Recombinant human CDC42EP4 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-356 aa of BC010451 Sequence: MLILKQLVSSSVHSKRRSRADLTAEMISAPLGDFRHTMHVGRAGDAFGDTSFLNSKAGEPDGESLDEQPSSSSSKRSLLSRKFRGSKRSQSVTRGEREQRDMLGSLRDSALFVKNAMSLPQLNEKEAAEKGTSKLPKSLSSSPVKKANDGEGGDEEAGTEEAVPRRNGAAGPHSPDPLLDEQAFGDLTDLPVVPKATYGLKHAESIMSFHIDLGPSMLGDVLSIMDKEEWDPEEGEGGYHGDEGAAGTITQAPPYAVAAPPLARQEGKAGPDLPSLPSHALEDEGWAAAAPSPGSARSMGSHTTRDSSSLSSCTSGILEERSPAFRGPDRARAAVSRQPDKEFSFMDEEEEDEIRV Predict reactive species\nFull Name: CDC42 effector protein (Rho GTPase binding) 4\nCalculated Molecular Weight: 38 kDa\nObserved Molecular Weight: 43-45 kDa\nGenBank Accession Number: BC010451\nGene Symbol: CDC42EP4\nGene ID (NCBI): 23580\nRRID: AB_10598005\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9H3Q1\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869654274217,"sku":"19262-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-19262-1-ap","provider":"Iright","version":"1.0","type":"link"}