{"product_id":"proteintech-19820-1-ap","title":"Proteintech, 19820-1-AP, MIS12 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe MIS12 (19820-1-AP) by Proteintech is a Polyclonal antibody targeting MIS12 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n19820-1-AP targets MIS12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, HeLa cells,  HepG2 cells\nPositive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunohistochemistry (IHC): IHC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe MIS12 complex, composed of the Mis12, Pmf1, Nsl1 and Dsn1 subunits, is a highly conserved kinetochore protein that interacts with the Ndc80 and Knl1 complexes, forming the KMN network (PMID: 32341029). MIS12 is a part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis. MIS12 is essential for proper kinetochore microtubule attachments (PubMed:23891108)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag13870 Product name: Recombinant human MIS12 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-205 aa of BC000229 Sequence: MSVDPMTYEAQFFGFTPQTCMLRIYIAFQDYLFEVMQAVEQVILKKLDGIPDCDISPVQIRKCTEKFLCFMKGHFDNLFSKMEQLFLQLILRIPSNILLPEDKCKETPYSEEDFQHLQKEIEQLQEKYKTELCTKQALLAELEEQKIVQAKLKQTLTFFDELHNVGRDHGTSDFRESLVSLVQNSRKLQNIRDNVEKESKRLKIS Predict reactive species\nFull Name: MIS12, MIND kinetochore complex component, homolog (yeast)\nCalculated Molecular Weight: 205 aa, 24 kDa\nObserved Molecular Weight: 25-28 kDa\nGenBank Accession Number: BC000229\nGene Symbol: MIS12\nGene ID (NCBI): 79003\nRRID: AB_3669315\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9H081\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887719993513,"sku":"19820-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-19820-1-ap","provider":"Iright","version":"1.0","type":"link"}