{"product_id":"proteintech-19979-1-ap","title":"Proteintech, 19979-1-AP, PAK2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PAK2 (19979-1-AP) by Proteintech is a Polyclonal antibody targeting PAK2 in WB, IF\/ICC, IP, ELISA applications with reactivity to human samples\n19979-1-AP targets PAK2 in WB, IF\/ICC, IP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, human skeletal muscle tissue,  Jurkat cells,  HeLa cells,  PC-3 cells,  Raji cells\nPositive IP detected in: K-562 cells\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:5000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPAK2, also named as PAK65, PAKgamma, p58, PAK-2p27, PAK-2p24 and C-t-PAK2, belongs to the protein kinase superfamily, STE Ser\/Thr protein kinase family and STE20 subfamily. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. PAK2 has several isoforms with the MW of 54-62 kDa and 41 kDa. The 62 kDa PAK2 is cleaved into a 34 kDa C terminal fragment and a 28 kDa N terminal fragment with a time course that parallels apoptotic death in Jurkat cells. (PMID:10200518). Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form. The antibody has no cross reaction to PAK1 and PAK3.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: p21 protein (Cdc42\/Rac)-activated kinase 2\nCalculated Molecular Weight: 58 kDa\nObserved Molecular Weight: 54 kDa\nGenBank Accession Number: NM_002577\nGene Symbol: PAK2\nGene ID (NCBI): 5062\nRRID: AB_10644144\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q13177\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869569994921,"sku":"19979-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-19979-1-ap","provider":"Iright","version":"1.0","type":"link"}