{"product_id":"proteintech-19987-1-ap","title":"Proteintech, 19987-1-AP, LDHA-Specific Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe LDHA-Specific (19987-1-AP) by Proteintech is a Polyclonal antibody targeting LDHA-Specific in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n19987-1-AP targets LDHA-Specific in WB, IHC, IF\/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, A431 cells,  HeLa cells,  MDA-MB-231 cells,  mouse testis tissue,  rat kidney tissue,  Jurkat cells,  NIH\/3T3 cells,  MCF-7 cells\nPositive IP detected in: mouse kidney tissue\nPositive IHC detected in: human heart tissue,  human skeletal muscle tissue,  human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nLactate dehydrogenase (LDH) is composed of A subunits predominate in skeletal muscle and B subunits are abundantly produced in brain and heart. The LDHA (lactate dehydrogenase A) and COPB1 (coatomer protein complex, subunit beta 1)genes, are involved in energy metabolism and protein transport processes. Both genes might play important roles in muscle development. It has some isoforms with the molecular mass of 27-40 kDa and can form a homotetramer(PMID:11276087). This antibody is specific to LDHA and has no cross reaction to LDHB and LDHC.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, rabbit, chicken, goat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: lactate dehydrogenase A\nCalculated Molecular Weight: 37 kDa\nObserved Molecular Weight: 32-37 kDa\nGenBank Accession Number: NM_005566\nGene Symbol: LDHA\nGene ID (NCBI): 3939\nRRID: AB_10646429\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P00338\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868821442729,"sku":"19987-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-19987-1-ap","provider":"Iright","version":"1.0","type":"link"}