{"product_id":"proteintech-20220-1-ap","title":"Proteintech, 20220-1-AP, ROBO3-Specific Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe ROBO3-Specific (20220-1-AP) by Proteintech is a Polyclonal antibody targeting ROBO3-Specific in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n20220-1-AP targets ROBO3-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Neuro-2a cells, mouse brain tissue,  rat brain tissue\nPositive IHC detected in: mouse brain tissue, human brain tissue,  human gliomas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nROBO3 belongs to the immunoglobulin superfamily and ROBO family. ROBO3 is thought to be involved during neural development in axonal navigation at the ventral midline of the neural tube. In spinal cord development, it plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1. It is required for hindbrain axon midline crossing. Defects in ROBO3 are a cause of familial horizontal gaze palsy with progressive scoliosis (HGPPS). Patients show a medulla where motor and sensory projections appear uncrossed. The antibody is specific to ROBO3.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: roundabout, axon guidance receptor, homolog 3 (Drosophila)\nCalculated Molecular Weight: 148 kDa\nObserved Molecular Weight: 148 kDa\nGenBank Accession Number: NM_022370\nGene Symbol: ROBO3\nGene ID (NCBI): 64221\nRRID: AB_10732825\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q96MS0\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869588181161,"sku":"20220-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-20220-1-ap","provider":"Iright","version":"1.0","type":"link"}