{"product_id":"proteintech-20233-1-ap","title":"Proteintech, 20233-1-AP, TOP2A-Specific Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe TOP2A-Specific (20233-1-AP) by Proteintech is a Polyclonal antibody targeting TOP2A-Specific in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human samples\n20233-1-AP targets TOP2A-Specific in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HepG2 cells,  L02 cells\nPositive IP detected in: L02 cells\nPositive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: U2OS cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:16000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:200-1:800\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nTOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL\/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a\/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: topoisomerase (DNA) II alpha 170kDa\nCalculated Molecular Weight: 174 kDa\nObserved Molecular Weight: 170-177 kDa\nGenBank Accession Number: NM_001067\nGene Symbol: TOP2A\nGene ID (NCBI): 7153\nRRID: AB_10664923\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P11388\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868866695337,"sku":"20233-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-20233-1-ap","provider":"Iright","version":"1.0","type":"link"}