{"product_id":"proteintech-20366-1-ap","title":"Proteintech, 20366-1-AP, SUZ12 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe SUZ12 (20366-1-AP) by Proteintech is a Polyclonal antibody targeting SUZ12 in WB, IHC, IP, ELISA applications with reactivity to human, rat samples\n20366-1-AP targets SUZ12 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, HeLa cells,  HepG2 cells,  MCF-7 cells\nPositive IP detected in: HeLa cells\nPositive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSUZ12, also termed  CHET9, JJAZ1 or KIAA0160, belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family. It is a polycomb group (PcG) protein. SUZ12 is a component of the PRC2\/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2\/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2\/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. A chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: suppressor of zeste 12 homolog (Drosophila)\nCalculated Molecular Weight: 83 kDa\nObserved Molecular Weight: 80-90 kDa\nGenBank Accession Number: NM_015355\nGene Symbol: SUZ12\nGene ID (NCBI): 23512\nRRID: AB_10694152\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q15022\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868880687273,"sku":"20366-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-20366-1-ap","provider":"Iright","version":"1.0","type":"link"}