{"product_id":"proteintech-20567-1-ap","title":"Proteintech, 20567-1-AP, DICER1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe DICER1 (20567-1-AP) by Proteintech is a Polyclonal antibody targeting DICER1 in WB, IHC, IF\/ICC, IF-P, FC (Intra), IP, ELISA applications with reactivity to human samples\n20567-1-AP targets DICER1 in WB, IHC, IF\/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, K-562 cells,  HepG2 cells\nPositive IP detected in: Jurkat cells\nPositive IHC detected in: human testis tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: human ovary tumor tissue\nPositive IF\/ICC detected in: HepG2 cells\nPositive FC (Intra) detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:200-1:1000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)-P: IF-P : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nDICER1, also named as DICER, HERNA and KIAA0928, belongs to the helicase family and Dicer subfamily. It is required for formation of the RNA induced silencing complex (RISC). DICER1 is a component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2\/AGO2 and TARBP2. Within the RLC\/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2\/AGO2. EIF2C2\/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. DICER1 cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs. The antibody is specific to DICER1. The calcualted molecular weight of DICER1 is 219 kDa, but modified DICER1 is about 220-250 kDa. DICER1 exists some isoforms with molecular weight 219 kDa and 93 kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: dicer 1, ribonuclease type III\nCalculated Molecular Weight: 219 kDa\nObserved Molecular Weight: 220-250 kDa, 90 kDa\nGenBank Accession Number: NM_030621\nGene Symbol: DICER1\nGene ID (NCBI): 23405\nRRID: AB_10694287\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9UPY3\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868869185705,"sku":"20567-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-20567-1-ap","provider":"Iright","version":"1.0","type":"link"}