{"product_id":"proteintech-20582-1-ap","title":"Proteintech, 20582-1-AP, PERK\/EIF2AK3 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PERK\/EIF2AK3 (20582-1-AP) by Proteintech is a Polyclonal antibody targeting PERK\/EIF2AK3 in WB, IHC, IF\/ICC, FC (Intra), ELISA applications with reactivity to human samples\n20582-1-AP targets PERK\/EIF2AK3 in WB, IHC, IF\/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, HepG2 cells,  MCF-7 cells\nPositive IHC detected in: human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HEK-293 cells\nPositive FC (Intra) detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPERK, also named as EIF2AK3 or PEK, belongs to the protein kinase superfamily, Ser\/Thr protein kinase family and GCN2 subfamily. It acts potentially as a metabolic sensor in the INS-secreting beta-cells to modulate the trafficking and quality control of pro-INS in the ER relative to the physiological demands for circulating INS (PMID:20530744). PERK and EIF2AK4 also have a functional role on regulating translation under non-stressed conditions, apart from their long established roles as stress kinases (PMID: 21638082). Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS). The molecular mass of PERK is 125 kDa and can be detected as 140 kDa or 170 kDa due to some modifications such as  glycosylation and phosphorylation (PMID: 20851775, 24371088, 21703327). This antibody is specific to PERK.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, pig, bovine\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: eukaryotic translation initiation factor 2-alpha kinase 3\nCalculated Molecular Weight: 125 kDa\nObserved Molecular Weight: 140 kDa\nGenBank Accession Number: NM_004836\nGene Symbol: PERK\nGene ID (NCBI): 9451\nRRID: AB_10695760\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9NZJ5\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868826620073,"sku":"20582-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-20582-1-ap","provider":"Iright","version":"1.0","type":"link"}