{"product_id":"proteintech-20654-1-ap","title":"Proteintech, 20654-1-AP, SMARCB1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe SMARCB1 (20654-1-AP) by Proteintech is a Polyclonal antibody targeting SMARCB1 in WB, IP, IHC, ELISA applications with reactivity to human samples\n20654-1-AP targets SMARCB1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HepG2 cells,  K-562 cells\nPositive IP detected in: K-562 cells\nPositive IHC detected in: human lymphoma tissue,  human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSMARCB1, also named as BAF47, INI1 and SNF5L1, belongs to the SNF5 family. It is a core component of the BAF (hSWI\/SNF) complex. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. SMARCB1 stimulates in vitro the remodeling activity of SMARCA4\/BRG1\/BAF190A. It is involved in activation of CSF1 promoter. SMARCB1 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem\/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. SMARCB1 plays a key role in cell-cycle control and causes cell cycle arrest in G0\/G1. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Defects in SMARCB1 are a cause of rhabdoid tumor (RDT) which also known as malignant rhabdoid tumor (MRT). Defects in SMARCB1 are a cause of schwannomatosis. The antibody is specific to SMARCB1.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: SWI\/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1\nCalculated Molecular Weight: 44 kDa\nObserved Molecular Weight: 40-45 kDa\nGenBank Accession Number: NM_003073\nGene Symbol: SMARCB1\nGene ID (NCBI): 6598\nRRID: AB_10695761\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q12824\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868989280425,"sku":"20654-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-20654-1-ap","provider":"Iright","version":"1.0","type":"link"}