{"product_id":"proteintech-20705-1-ap","title":"Proteintech, 20705-1-AP, TOP1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe TOP1 (20705-1-AP) by Proteintech is a Polyclonal antibody targeting TOP1 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human samples\n20705-1-AP targets TOP1 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: L02 cells\nPositive IHC detected in: human stomach cancer tissue, human colon cancer tissue,  human cervical cancer tissue,  human ovary tumor tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:300-1:1200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nTOP1 belongs to the eukaryotic type I topoisomerase family. The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. The antibody is specific to TOP1.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: topoisomerase (DNA) I\nCalculated Molecular Weight: 91 kDa\nObserved Molecular Weight: 90 kDa\nGenBank Accession Number: NM_003286\nGene Symbol: TOP1\nGene ID (NCBI): 7150\nRRID: AB_10695762\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P11387\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868883243177,"sku":"20705-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-20705-1-ap","provider":"Iright","version":"1.0","type":"link"}