{"product_id":"proteintech-20962-1-ap","title":"Proteintech, 20962-1-AP, PYCR1-specific Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PYCR1-specific (20962-1-AP) by Proteintech is a Polyclonal antibody targeting PYCR1-specific in WB, IHC, IP, ELISA applications with reactivity to human, mouse samples\n20962-1-AP targets PYCR1-specific in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Raji cells, FaDu cells,  human brain tissue,  HepG2 cells,  MCF-7 cells,  Ramos cells,  U2OS cells\nPositive IP detected in: Raji cells\nPositive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa. The MW of PYCR1 is about 33-35kd. This antibody is specific to PYCR1.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: pyrroline-5-carboxylate reductase 1\nCalculated Molecular Weight: 33 kDa\nObserved Molecular Weight: 33-35 kDa\nGenBank Accession Number: NM_006907\nGene Symbol: PYCR1\nGene ID (NCBI): 5831\nRRID: AB_10638486\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P32322\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869492007081,"sku":"20962-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-20962-1-ap","provider":"Iright","version":"1.0","type":"link"}