{"product_id":"proteintech-21069-1-ap","title":"Proteintech, 21069-1-AP, SIM2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe SIM2 (21069-1-AP) by Proteintech is a Polyclonal antibody targeting SIM2 in WB, IHC, IP, ELISA applications with reactivity to human, mouse samples\n21069-1-AP targets SIM2 in WB, IHC, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: COLO 320 cells, human skeletal muscle tissue,  HeLa cells,  HepG2 cells,  U-251 cells\nPositive IP detected in: mouse skeletal muscle tissue\nPositive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:3000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSIM2, also named as single-minded homolog 2, is a 667 amino acid protein, which contains 1 bHLH (basic helix-loop-helix) domain and one PAS-associated C-terminal domain. SIM2 localizes in the nucleus and has tow isoforms. Isoform SIM2 is a 73 kDa protein and Isoform SIM2S is 63 kDa protein. SIM2 as a transcription factor may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag15204 Product name: Recombinant human SIM2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 342-525 aa of BC110444 Sequence: LSLEQVSTAKSQDSWRTALSTSQETRKLVKPKNTKMKTKLRTNPYPPQQYSSFQMDKLECGQLGNWRASPPASAAAPPELQPHSESSDLLYTPSYSLPFSYHYGHFPLDSHVFSSKKPMLPAKFGQPQGSPCEVARFFLSTLPASGECQWHYANPLVPSSSSPAKNPPEPPANTARHSLVPSYE Predict reactive species\nFull Name: single-minded homolog 2 (Drosophila)\nCalculated Molecular Weight: 667 aa, 73 kDa\nObserved Molecular Weight: 60-64 kDa\nGenBank Accession Number: BC110444\nGene Symbol: SIM2\nGene ID (NCBI): 6493\nRRID: AB_10732844\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q14190\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869764112553,"sku":"21069-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-21069-1-ap","provider":"Iright","version":"1.0","type":"link"}