{"product_id":"proteintech-21302-1-ap","title":"Proteintech, 21302-1-AP, JAML Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe JAML (21302-1-AP) by Proteintech is a Polyclonal antibody targeting JAML in WB, IHC, ELISA applications with reactivity to human samples\n21302-1-AP targets JAML in WB, IHC, IF, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HL-60 cells, Jurkat cells,  L02 cells\nPositive IHC detected in: human spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunohistochemistry (IHC): IHC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nJunctional adhesion molecules (JAM-A, JAM-B, and JAM-C) are endothelial and epithelial adhesion molecules involved in the recruitment of circulating leukocytes to inflammatory sites (PMID: 19064666). JAML (junctional adhesion molecule-like), also known as AMICA1, is related to JAM proteins. It contains 2 extracellular immunoglobulin-like domains, a transmembrane segment, and a cytoplasmic tail. JAML is expressed on leukocytes and promotes their adhesion to endothelial cells (PMID: 12869515). JAML can regulate neutrophil transepithelial migration via binding interactions with epithelial coxsackie and adenovirus receptor (CAR) (PMID: 15800062). The full-length human JAML protein has a calculated molecular weight of 44 kDa. This antibody raised against 40-275 aa of human JAML protein detects bands with molecular wight of 44 kDa, and 50-70 kDa which probably represent different glycoforms of the protein (PMID: 12869515; 24621992; 15800062).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag15900 Product name: Recombinant human AMICA1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 40-275 aa of BC100797 Sequence: VPIGRFQNRVHLMGDILCNDGSLLLQDVQEADQGTYICEIRLKGESQVFKKAVVLHVLPEEPKELMVHVGGLIQMGCVFQSTEVKHVTKVEWIFSGRRAKEEIVFRYYHKLRMSVEYSQSWGHFQNRVNLVGDIFRNDGSIMLQGVRESDGGNYTCSIHLGNLVFKKTIVLHVSPEEPRTLVTPAALRPLVLGGNQLVIIVGIVCATILLLPVLILIVKKTCGNKSSVNSTVLVKN Predict reactive species\nFull Name: adhesion molecule, interacts with CXADR antigen 1\nCalculated Molecular Weight: 394 aa, 44 kDa\nObserved Molecular Weight: 44 kDa, 50-70 kDa\nGenBank Accession Number: BC100797\nGene Symbol: JAML\nGene ID (NCBI): 120425\nRRID: AB_10733103\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q86YT9\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869413462185,"sku":"21302-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-21302-1-ap","provider":"Iright","version":"1.0","type":"link"}