{"product_id":"proteintech-21468-1-ap","title":"Proteintech, 21468-1-AP, XRCC1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe XRCC1 (21468-1-AP) by Proteintech is a Polyclonal antibody targeting XRCC1 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human samples\n21468-1-AP targets XRCC1 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, K-562 cells,  Jurkat cells\nPositive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: A431 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:12000\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nXRCC1 (X-ray Repair Cross Complementing group 1) is a molecular scaffold protein that interacts with multiple enzymatic components of DNA single-strand break repair (SSBR) including DNA kinase, DNA phosphatase, DNA polymerase, DNA deadenylase, and DNA ligase activities that collectively are capable of accelerating the repair of a broad range of DNA single-strand breaks (SSBs) (PMID: 18515191). XRCC1 is 633 amino acids in length which is comprised of an N-terminal domain (NTD) of ˜160 amino acids that interacts with Pol β, a central BRCT domain of ˜90 amino acids that interacts with PARP1 (PMID: 9584196). This antibody is not suitable for murine samples.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag15172 Product name: Recombinant human XRCC1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 284-633 aa of BC023593 Sequence: TAPVPARAQGAVTGKPRGEGTEPRRPRAGPEELGKILQGVVVVLSGFQNPFRSELRDKALELGAKYRPDWTRDSTHLICAFANTPKYSQVLGLGGRIVRKEWVLDCHRMRRRLPSQRYLMAGPGSSSEEDEASHSGGSGDEAPKLPQKQPQTKTKPTQAAGPSSPQKPPTPEETKAASPVLQEDIDIEGVQSEGQDNGAEDSGDTEDELRRVAEQKEHRLPPGQEENGEDPYAGSTDENTDSEEHQEPPDLPVPELPDFFQGKHFFLYGEFPGDERRKLIRYVTAFNGELEDYMSDRVQFVITAQEWDPSFEEALMDNPSLAFVRPRWIYSCNEKQKLLPHQLYGVVPQA Predict reactive species\nFull Name: X-ray repair complementing defective repair in Chinese hamster cells 1\nCalculated Molecular Weight: 633 aa, 70 kDa\nObserved Molecular Weight: 80-90 kDa\nGenBank Accession Number: BC023593\nGene Symbol: XRCC1\nGene ID (NCBI): 7515\nRRID: AB_2878865\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P18887\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868922302633,"sku":"21468-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-21468-1-ap","provider":"Iright","version":"1.0","type":"link"}