{"product_id":"proteintech-21499-1-ap","title":"Proteintech, 21499-1-AP, SH3D19 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe SH3D19 (21499-1-AP) by Proteintech is a Polyclonal antibody targeting SH3D19 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n21499-1-AP targets SH3D19 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HT-1080 cells,  Y79 cells\nPositive IHC detected in: mouse heart tissue, human heart tissue,  human kidney tissue,  mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: A431 cells, HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunohistochemistry (IHC): IHC : 1:200-1:800\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSH3D19, also known as EBP, Eve-1, encodes a multiple SH3 domain-containing protein. SH3D19 plays a role in regulating A disintegrin and metalloproteases (ADAMs) in the signaling of EGFR-ligand shedding. SH3D19 is involved in the suppression of Ras-induced cellular transformation and Ras-mediated activation of ELK1(PMID: 14551139). SH3D19 is abundantly expressed in skeletal muscle and heart(PMID: 15280379).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag15915 Product name: Recombinant human SH3D19 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 415-764 aa of BC108890 Sequence: VLVMLKQTENNYLECQKGEDTGRVHLSQMKIITPLDEHLRSRPNDPSHAQKPVDSGAPHAVVLHDFPAEQVDDLNLTSGEIVYLLEKIDTDWYRGNCRNQIGIFPANYVKVIIDIPEGGNGKRECVSSHCVKGSRCVARFEYIGEQKDELSFSEGEIIILKEYVNEEWARGEVRGRTGIFPLNFVEPVEDYPTSGANVLSTKVPLKTKKEDSGSNSQVNSLPAEWCEALHSFTAETSDDLSFKRGDRIQILERLDSDWCRGRLQDREGIFPAVFVRPCPAEAKSMLAIVPKGRKAKALYDFRGENEDELSFKAGDIITELESVDDDWMSGELMGKSGIFPKNYIQFLQIS Predict reactive species\nFull Name: SH3 domain containing 19\nCalculated Molecular Weight: 790 aa, 87 kDa\nObserved Molecular Weight: 45-47 kDa\nGenBank Accession Number: BC108890\nGene Symbol: SH3D19\nGene ID (NCBI): 152503\nRRID: AB_10733218\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q5HYK7\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887800176809,"sku":"21499-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-21499-1-ap","provider":"Iright","version":"1.0","type":"link"}