{"product_id":"proteintech-21652-1-ap","title":"Proteintech, 21652-1-AP, Cardiac Troponin I Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe Cardiac Troponin I (21652-1-AP) by Proteintech is a Polyclonal antibody targeting Cardiac Troponin I in WB, IHC, IF-P, IP, ELISA applications with reactivity to human, mouse, rat samples\n21652-1-AP targets Cardiac Troponin I in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse heart tissue, rat heart tissue,  HEK-293 cells\nPositive IP detected in: mouse heart tissue\nPositive IHC detected in: mouse heart tissue, human heart tissue,  mouse lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: mouse heart tissue\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:20000-1:100000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nImmunofluorescence (IF)-P: IF-P : 1:300-1:1200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nTroponin I (TnI) is the inhibitory subunit of troponin; the thin filament regulatory complex that confers calcium sensitivity to striated muscle actomyosin ATPase activity. Cardiac Troponin I (cTnI) is encoded by theTNNI3gene located on chromosome 19 in humans.What is the molecular weight of cTnI?The molecular weight of cTnI is 24 kDa.What is the tissue specificity of cTnI?cTnI is produced exclusively by cardiac muscle.What are the post-translational modifications of cTnI?cTnI at Ser-23 and Ser-24 under adrenergic stimulation enhances the relaxation of cardiac muscle.What is the role of cTnI in disease?cTnI is considered to be one of the most specific and sensitive markers of myocardial damage such as acute myocardial infarction (AMI), as detection of cTnI in serum is widely accepted as a diagnostic tool (PMID: 15452154). Defects inTNNI3lead to cardiomyopathy familial hypertrophic type 7 (CMH7); an inherited heart disorder characterized by ventricular hypertrophy (PMID: 8205619). Defects inTNNI3can also cause cardiomyopathy familial restrictive type I (RCM1) (PMID: 20617149) and cardiomyopathy dilated type 2A (CMD2A) (PMID: 12531876). These disorders are characterized by the impaired filling of ventricles or ventricular dilation respectively.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag16365 Product name: Recombinant human TNNI3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-110 aa of BC096165 Sequence: MADGSSDAAREPRPAPAPIRRRSSNYRAYATEPHAKKKSKISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEE Predict reactive species\nFull Name: troponin I type 3 (cardiac)\nCalculated Molecular Weight: 210 aa, 24 kDa\nObserved Molecular Weight: 24-26 kDa\nGenBank Accession Number: BC096165\nGene Symbol: Cardiac Troponin I\nGene ID (NCBI): 7137\nRRID: AB_2878898\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P19429\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868848935081,"sku":"21652-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-21652-1-ap","provider":"Iright","version":"1.0","type":"link"}