{"product_id":"proteintech-21860-1-ap","title":"Proteintech, 21860-1-AP, Cyclin T2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe Cyclin T2 (21860-1-AP) by Proteintech is a Polyclonal antibody targeting Cyclin T2 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n21860-1-AP targets Cyclin T2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HepG2 cells, Jurkat cells,  NIH\/3T3 cells\nPositive IHC detected in: mouse brain tissue, rat brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCyclin T2 (CCNT2) belongs to the cyclin family. CCNT2 is regulatory subunit of the cyclin-dependent kinase pair (CDK9\/cyclin T) complex, also called positive transcription elongation factor B (P-TEFB), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNAP II)  (PMID: 9499409, PMID: 15563843). Cyclin T2 is expressed at a relatively high level in freshly isolated monocytes and its level remains constant when the cells are induced to undergo macrophage differentiation (PMID: 21791050). In contrast, Cyclin T1 is expressed at low levels in monocytes and it is strongly up-regulated by a post-transcriptional mechanism when the cells are induced to differentiate to macrophages.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag16514 Product name: Recombinant human CCNT2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 251-610 aa of BC114366 Sequence: LKKIRNWRANQAARKPKVDGQVSETPLLGSSLVQNSILVDSVTGVPTNPSFQKPSTSAFPAPVPLNSGNISVQDSHTSDNLSMLATGMPSTSYGLSSHQEWPQHQDSARTEQLYSQKQETSLSGSQYNINFQQGPSISLHSGLHHRPDKISDHSSVKQEYTHKAGSSKHHGPISTTPGIIPQKMSLDKYREKRKLETLDLDVRDHYIAAQVEQQHKQGQSQAASSSSVTSPIKMKIPIANTEKYMADKKEKSGSLKLRIPIPPTDKSASKEELKMKIKVSSSERHSSSDEGSGKSKHSSPHISRDHKEKHKEHPSSRHHTSSHKHSHSHSGSSSGGSKHSADGIPPTVLRSPVGLSSDGI Predict reactive species\nFull Name: cyclin T2\nCalculated Molecular Weight: 730 aa, 81 kDa\nObserved Molecular Weight: 70 kDa\nGenBank Accession Number: BC114366\nGene Symbol: Cyclin T2\nGene ID (NCBI): 905\nRRID: AB_3085675\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O60583\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869667840169,"sku":"21860-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-21860-1-ap","provider":"Iright","version":"1.0","type":"link"}