{"product_id":"proteintech-21891-1-ap","title":"Proteintech, 21891-1-AP, P53 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe P53 (21891-1-AP) by Proteintech is a Polyclonal antibody targeting P53 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human samples\n21891-1-AP targets P53 in WB, IHC, IF\/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, A431 cells,  HT-29 cells\nPositive IP detected in: A431 cells\nPositive IHC detected in: human prostate cancer tissue, human gliomas tissue,  human endometrial cancer tissue,  human ovary tumor tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\n1.       What is p53?P53 is a tumor suppressor gene that plays a role in maintaining genomic stability and controlling apoptosis. During the cell cycle, it can arrest cells at the G1\/S checkpoint and activate DNA repair mechanisms. It is the most mutated gene in cancer. In unstressed cells, p53 usually exists at low levels in an inactive form, being bound to Mdm2.2.       FAQs and p53a.I fail to detect p53 by western blottingBasal levels of wild-type p53 in untreated cells can be low. Try to load more cell lysate and use a positive control - a lysate of cells treated with DNA-damaging agents should increase p53 levels.b.I fail to detect p53 in some cell lines by western blottingVarious p53 mutations are present in cancer cell types. If mutations cause truncations\/deletions some monoclonal antibodies may no longer recognize mutated p53. You have more chances of detecting various p53 mutants with our polyclonal antibody.c.I can detect more than one band ~50 kDa size \/ different cell lines give bands at slightly different sizep53 is a subject of post-translational modifications (http:\/\/p53.free.fr\/p53_info\/p53_modifications.html) and more than one isoform may be expressed (http:\/\/p53.free.fr\/p53_info\/p53_isoforms.html). Also, it is possible that your cell line of interest expresses one allele with mutated p53 with altered molecular weight.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag16596 Product name: Recombinant human TP53 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-393 aa of BC003596 Sequence: MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPRVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACAGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD Predict reactive species\nFull Name: tumor protein p53\nCalculated Molecular Weight: 393 aa, 44 kDa\nObserved Molecular Weight: 53 kDa\nGenBank Accession Number: BC003596\nGene Symbol: P53\nGene ID (NCBI): 7157\nRRID: AB_10896826\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P04637\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868836384937,"sku":"21891-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-21891-1-ap","provider":"Iright","version":"1.0","type":"link"}