{"product_id":"proteintech-22050-1-ap","title":"Proteintech, 22050-1-AP, CEP97 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe CEP97 (22050-1-AP) by Proteintech is a Polyclonal antibody targeting CEP97 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, canine samples\n22050-1-AP targets CEP97 in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, canine samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HL-60 cells,  HEK-293 cells,  HeLa cells,  PC-3 cells\nPositive IP detected in: HeLa cells\nPositive IHC detected in: human breast cancer tissue,  human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: MDCK cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:500-1:2000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCEP97, also named as  LRRIQ2, contains one IQ domain, eight LRR (leucine-rich) repeats and one LRRCT domain. It collaborates with CEP110, being involved in the suppression of a cilia assembly program. CEP97 is required for correct spindle formation and has a role in cytokinesis. It is required for recruitment of CEP110 to the centrosome. This protein has a calculated MW  ~97 kDa, although the protein migrates at a molecular weight of approximate 140 kDa. The protein is highly acidic, with a pI ~4.7, providing a potential explanation for its reduced mobility in SDS-PAGE. [PMID: 17719545]. This is a rabbit polyclonal antibody raised against the C terminus of human CEP97.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, canine\nCited Reactivity: human, mouse, drosophila\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag16995 Product name: Recombinant human CEP97 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 506-865 aa of BC041085 Sequence: ESTEQKQSDIKKPENTQPENKETISQATSEKLPMILTQRSVALGQDKVALQKLNDAATKLQACWRGFYARNYNPQAKDVRYEIRLRRMQEHIVCLTDEIRRLRKERDEERIKKFVQEEAFRFLWNQVRSLQVWQQTVDQRLSSWHTDVPPISSTLVPSKHPLFTQSQESSCDQNADWFIASDVAPQEKSLPEFPDSGFHSSLTEQVHSLQHSLDFEKSSTEGSESSIMGNSIDTVRYGKESDLGDVSEEHGEWNKESSNNEQDNSLLEQYLTSVQQLEDADERTNFDTETRDSKLHIACFPVQLDTLSDGASVDESHGISPPLQGEISQTQENSKLNAEVQGQQPECDSTFQLLHVGVTV Predict reactive species\nFull Name: centrosomal protein 97kDa\nCalculated Molecular Weight: 865 aa, 97 kDa\nObserved Molecular Weight: 130-140 kDa\nGenBank Accession Number: BC041085\nGene Symbol: CEP97\nGene ID (NCBI): 79598\nRRID: AB_11182378\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q8IW35\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868867449001,"sku":"22050-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-22050-1-ap","provider":"Iright","version":"1.0","type":"link"}