{"product_id":"proteintech-22280-1-ap","title":"Proteintech, 22280-1-AP, MYH7-specific Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe MYH7-specific (22280-1-AP) by Proteintech is a Polyclonal antibody targeting MYH7-specific in WB, IHC, IF\/ICC, IF-P, IP, ELISA applications with reactivity to human, mouse, rat samples\n22280-1-AP targets MYH7-specific in WB, IHC, IF\/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse heart tissue, rat heart tissue\nPositive IP detected in: mouse heart tissue\nPositive IHC detected in: mouse skeletal muscle tissue, human skeletal muscle tissue,  mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: mouse heart tissue\nPositive IF\/ICC detected in: H9C2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)-P: IF-P : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:300-1:1200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMyosin is the major contractile protein of all eukaryotic cells, existing as a hexameric molecule composed of two myosin heavy chains (MHCs) that contain the catalytic sites for ATP hydrolysis, and four lower molecular weight myosin light chains. MYH7 encodes the slow\/beta-cardiac myosin heavy chain (MHC), which is the major MHC isoform of cardiac ventricles. It is also expressed in slow, oxidative, type 1 muscle fibers of skeletal muscle. Numerous mutations of MYH7 have been linked to familial hypertrophic cardiomyopathy. This antibody can specifically recognize endogenous MYH7, not cross-reacting with other MHCs (PMID: 2523412, 14520662). Due to the production of Heavy Meromyosin (HMM) during proteolysis of myosin, a band around 150 kDa may be detected, which may correspond to the HMM part of MYH7 (PMID: 14613868).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, pig, zebrafish, goat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: myosin, heavy chain 7, cardiac muscle, beta\nCalculated Molecular Weight: 223 kDa\nObserved Molecular Weight: 200-220 kDa\nGenBank Accession Number: NM_000257\nGene Symbol: MYH7\nGene ID (NCBI): 4625\nRRID: AB_2736821\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P12883\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868831109289,"sku":"22280-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-22280-1-ap","provider":"Iright","version":"1.0","type":"link"}