{"product_id":"proteintech-22676-1-ap","title":"Proteintech, 22676-1-AP, PPP4R2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PPP4R2 (22676-1-AP) by Proteintech is a Polyclonal antibody targeting PPP4R2 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human samples\n22676-1-AP targets PPP4R2 in WB, IHC, IF\/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A549 cells,  HepG2 cells\nPositive IP detected in: HepG2 cells\nPositive IHC detected in: human liver cancer tissue,  human pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPPP4R2 is a regulatory subunit of protein phosphatase-4 (PPP4), a protein serine\/threonine phosphatase implicated in microtubule organization at centrosomes and DNA double-strand break repair. The deduced 453-amino acid protein contains several potential phosphorylation sites and has a calculated molecular mass of 50.4 kD. Immunofluorescent analysis of HeLa and A431 cells revealed prominent localization of PPP4R2 at centrosomes, with weaker staining of cytoplasm and nucleus(PMID: 10769191).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag18486 Product name: Recombinant human PPP4R2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-205 aa of BC110889 Sequence: MDVERLQEALKDFEKRGKKEVCPVLDQFLCHVAKTGETIIPFTIQRLCELLTDPRRNYTGTDKFLRGVEKNVMVVSCVYPSSEKNNSNSLNRMNGVMFPGNSPSYTERSNINGPGTPRPLNRPKVSLSAPMTTNGLPESTDSKEANLQQNEEKNHSDSSTSESEVSSVSPLKNKHPDEDAVEAEGHEVKRLRFDKEGEVRETASQ Predict reactive species\nFull Name: protein phosphatase 4, regulatory subunit 2\nCalculated Molecular Weight: 360aa,40 kDa; 495aa,55 kDa\nObserved Molecular Weight: 65 kDa\nGenBank Accession Number: BC110889\nGene Symbol: PPP4R2\nGene ID (NCBI): 151987\nRRID: AB_2879149\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9NY27\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869735735465,"sku":"22676-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-22676-1-ap","provider":"Iright","version":"1.0","type":"link"}