{"product_id":"proteintech-23875-1-ap","title":"Proteintech, 23875-1-AP, SNRNP200 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe SNRNP200 (23875-1-AP) by Proteintech is a Polyclonal antibody targeting SNRNP200 in WB, IHC, FC (Intra), IP, ELISA applications with reactivity to human, mouse samples\n23875-1-AP targets SNRNP200 in WB, IHC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HEK-293 cells,  Jurkat cells,  NIH\/3T3 cells\nPositive IP detected in: HeLa cells\nPositive IHC detected in: human placenta tissue, mouse bladder tissue,  mouse testis tissue,  rat stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive FC (Intra) detected in: A431 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSNRNP200 (small nuclear ribonucleoprotein 200kDa (U5)), also known as HELIC2, ASCC3L1 or BRR2, is a 2,136 amino acid protein that localizes to the nucleus and contains two SEC63 domains, two helicase C-terminal domains and two helicase ATP-binding domains. Existing as multiple alternatively spliced isoforms, HELIC2 functions as an RNA helicase that is thought to promote specific RNA-RNA conformational changes which are important in the second step of RNA splicing. The gene encoding HELIC2 maps to human chromosome 2, which houses over 1,400 genes and comprises nearly 8% of the human genome. Harlequin icthyosis, a rare and morbid skin deformity, is associated \n\nwith mutations in the chromosome 2-localized ABCA12 gene, while the lipid metabolic disorder sitosterolemia is associated with defects in the ABCG5 and ABCG8 genes, hich also map to chromosome 2. The observed molecular weight of SNRNP200 is 200-245kd.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag20748 Product name: Recombinant human SNRNP200 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 1787-2136 aa of BC001417 Sequence: ELVEQTLSDLEQSKCISIEDEMDVAPLNLGMIAAYYYINYTTIELFSMSLNAKTKVRGLIEIISNAAEYENIPIRHHEDNLLRQLAQKVPHKLNNPKFNDPHVKTNLLLQAHLSRMQLSAELQSDTEEILSKAIRLIQACVDVLSSNGWLSPALAAMELAQMVTQAMWSKDSYLKQLPHFTSEHIKRCTDKGVESVFDIMEMEDEERNALLQLTDSQIADVARFCNRYPNIELSYEVVDKDSIRSGGPVVVLVQLEREEEVTGPVIAPLFPQKREEGWWVVIGDAKSNSLISIKRLTLQQKAKVKLDFVAPATGAHNYTLYFMSDAYMGCDQEYKFSVDVKEAETDSDSD Predict reactive species\nFull Name: small nuclear ribonucleoprotein 200kDa (U5)\nCalculated Molecular Weight: 494 aa, 57 kDa\nObserved Molecular Weight: 200-245 kDa\nGenBank Accession Number: BC001417\nGene Symbol: SNRNP200\nGene ID (NCBI): 23020\nRRID: AB_2879346\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen Affinity purified\nUNIPROT ID: O75643\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868989477033,"sku":"23875-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-23875-1-ap","provider":"Iright","version":"1.0","type":"link"}