{"product_id":"proteintech-24017-1-ap","title":"Proteintech, 24017-1-AP, LIN28B Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe LIN28B (24017-1-AP) by Proteintech is a Polyclonal antibody targeting LIN28B in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse samples\n24017-1-AP targets LIN28B in WB, IHC, IF\/ICC, IP, ELISA, PLA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, HepG2 cells,  K-562 cells\nPositive IP detected in: HEK-293 cells\nPositive IHC detected in: human ovary tumor tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: NCCIT cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:3000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nLIN28B, also named as CSDD2, belongs to the lin-28 family. It acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7). LIN2B is specifically expressed in embryonic muscle, neurons, and epithelia, as well as in embryonic carcinoma cells. However, it is suppressed in most adult tissues except for skeletal and cardiac muscle. The calculated molecular weight of LIN28B is 27 kDa, but a 35 kDa band has been detected in some articles (PMID: 33029224, 33378003).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag21241 Product name: Recombinant human LIN28B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 94-250 aa of BC137526 Sequence: LESIRVTGPGGSPCLGSERRPKGKTLQKRKPKGDRCYNCGGLDHHAKECSLPPQPKKCHYCQSIMHMVANCPHKNVAQPPASSQGRQEAESQPCTSTLPREVGGGHGCTSPPFPQEARAEISERSGRSPQEASSTKSSIAPEEQSKKGPSVQKRKKT Predict reactive species\nFull Name: lin-28 homolog B (C. elegans)\nCalculated Molecular Weight: 250 aa, 27 kDa\nObserved Molecular Weight: 35 kDa\nGenBank Accession Number: BC137526\nGene Symbol: LIN28B\nGene ID (NCBI): 389421\nRRID: AB_2879403\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q6ZN17\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869001765033,"sku":"24017-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-24017-1-ap","provider":"Iright","version":"1.0","type":"link"}