{"product_id":"proteintech-24337-1-ap","title":"Proteintech, 24337-1-AP, BBOF1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe BBOF1 (24337-1-AP) by Proteintech is a Polyclonal antibody targeting BBOF1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n24337-1-AP targets BBOF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse testis tissue, TT cells,  rat testis tissue\nPositive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nBasal body orientation factor 1 (BBOF1), also known as CCDC176, contains two coiled-coil domains and is evolutionarily conserved in vertebrates.  Recently, Xenopus bbof1 has been characterized as a cilia-associated factor that is induced by the transcription factor foxj1 to mediate the alignment and orientation of cilia in epidermal multi-ciliated cells. BBOF1 is required for sperm flagellar axoneme maintenance and male fertility in mice. BBOF1 expression is elevated during meiosis, and it is accumulated in round spermatids. BBOF1 deletion has compromised effects on growth, survival, and spermatogenesis.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag19302 Product name: Recombinant human C14orf45 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 12-229 aa of BC104981 Sequence: LQQHAMIENQAGQVEIDKLQHLLQMKDREMNRVKKLAKNILDERTEVERFFLDALHQVKQQILISRKHYKQIAQAAFNLKMRAACTGRTEYPKIRTFDGREHSTNSVNQDLLEAEKWTHIEGNVDIGDLTWEQKEKVLRLLFAKMNGCPSRKYNQSSRPPVPDYVVSDSGETKEFGDESKLQDKIFITQQIAISDSSGEVVLPTIPKEPQESDTGTF Predict reactive species\nFull Name: chromosome 14 open reading frame 45\nCalculated Molecular Weight: 529 aa, 62 kDa\nObserved Molecular Weight: 62 kDa\nGenBank Accession Number: BC104981\nGene Symbol: BBOF1\nGene ID (NCBI): 80127\nRRID: AB_3669433\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q8ND07\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46885189386409,"sku":"24337-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-24337-1-ap","provider":"Iright","version":"1.0","type":"link"}