{"product_id":"proteintech-25079-1-ap","title":"Proteintech, 25079-1-AP, GNE Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe GNE (25079-1-AP) by Proteintech is a Polyclonal antibody targeting GNE in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples\n25079-1-AP targets GNE in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse liver tissue, Jurkat cells,  RAW 264.7 cells,  rat liver tissue\nPositive IP detected in: Jurkat cells\nPositive IHC detected in: human kidney tissue,  human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:5000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe bifunctional enzyme UDP-N-acetylglucosamine-2-epimerase\/N-acetylmannosamine kinase (GNE) is essential for early embryonic development and catalyzes the rate limiting step in sialic acid biosynthesis. It plays an important role in neuronal cell and brain development, and is strongly involved in cardiac tissue and skeletal muscle early survival and organization.. This protein has 5 isoforms produced by alternative splicing.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag18689 Product name: Recombinant human GNE protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 376-722 aa of BC121179 Sequence: SIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPREGIVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAALAERKFGQGKGLENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQAAKLGNAKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIY Predict reactive species\nFull Name: glucosamine (UDP-N-acetyl)-2-epimerase\/N-acetylmannosamine kinase\nCalculated Molecular Weight: 722 aa, 79 kDa\nObserved Molecular Weight: 79 kDa\nGenBank Accession Number: BC121179\nGene Symbol: GNE\nGene ID (NCBI): 10020\nRRID: AB_2879887\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9Y223\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869408907433,"sku":"25079-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-25079-1-ap","provider":"Iright","version":"1.0","type":"link"}