{"product_id":"proteintech-25128-1-ap","title":"Proteintech, 25128-1-AP, Cleaved Caspase 3 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe Cleaved Caspase 3 (25128-1-AP) by Proteintech is a Polyclonal antibody targeting Cleaved Caspase 3 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse samples\n25128-1-AP targets Cleaved Caspase 3 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Jurkat cells\nPositive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCaspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can form heterocomplex with other proteins. This antibody can recognize the cleaved Caspase 3 fragments. This antibody is specific for cleaved caspase 3, and does not recognize full length caspase-3. The cleaved fragment of Caspase 3 might form complex and shows at around 30-35 kDa by western blot (PMID: 25501826).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse, rat, chicken, bovine, goat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: caspase 3, apoptosis-related cysteine peptidase\nCalculated Molecular Weight: 32 kDa\nObserved Molecular Weight: 17-25 kDa\nGenBank Accession Number: NM_032991\nGene Symbol: Caspase 3\nGene ID (NCBI): 836\nRRID: AB_3073913\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P42574\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868815708329,"sku":"25128-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-25128-1-ap","provider":"Iright","version":"1.0","type":"link"}