{"product_id":"proteintech-25149-1-ap","title":"Proteintech, 25149-1-AP, NAT12 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe NAT12 (25149-1-AP) by Proteintech is a Polyclonal antibody targeting NAT12 in WB, IF\/ICC, ELISA applications with reactivity to human samples\n25149-1-AP targets NAT12 in WB, IF\/ICC, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells\nPositive IF\/ICC detected in: U2OS cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nN-terminal acetylation (Nt-acetylation) by N-terminal acetyltransferases (NATs) is one of the most common protein modifications in eukaryotes. The NatC complex represents one of three major NATs of which the substrate profile remains largely unexplored. NAT12\/NAA30 (N-alpha-acetyltransferase 30) is the catalytic subunit of the NAT-C complex (PMID: 27694331, 26292663). The expected sizes of the two isoforms of the protein were 35 and 39 kDa. Western blots detected two bands of NAA12, the expected ~40 kDa band and a possibly dimer at ~70 kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag18435 Product name: Recombinant human NAT12 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-362 aa of BC118589 Sequence: MAEVPPGPSSLLPPPAPPAPAAVEPRCPFPAGAALACCSEDEEDDEEHEGGGSRSPAGGESATVAAKGHPCLRCPQPPQEQQQLNGLISPELRHLRAAASLKSKVLSVAEVAATTATPDGGPRATATKGAGVHSGERPPHSLSSNARTAVPSPVEAAAASDPAAARNGLAEGTEQEEEEEDEQVRLLSSSLTADCSLRSPSGREVEPGEDRTIRYVRYESELQMPDIMRLITKDLSEPYSIYTYRYFIHNWPQLCFLAMVGEECVGAIVCKLDMHKKMFRRGYIAMLAVDSKYRRNGIGTNLVKKAIYAMVEGDCDEVVLETEITNKSALKLYENLGFVRDKRLFRYYLNGVDALRLKLWLR Predict reactive species\nFull Name: N-acetyltransferase 12 (GCN5-related, putative)\nCalculated Molecular Weight: 362 aa, 39 kDa\nObserved Molecular Weight: 40 kDa\nGenBank Accession Number: BC118589\nGene Symbol: NAT12\nGene ID (NCBI): 122830\nRRID: AB_3085773\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q147X3\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887732707497,"sku":"25149-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-25149-1-ap","provider":"Iright","version":"1.0","type":"link"}