{"product_id":"proteintech-25566-1-ap","title":"Proteintech, 25566-1-AP, ALKBH8 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe ALKBH8 (25566-1-AP) by Proteintech is a Polyclonal antibody targeting ALKBH8 in IHC, IF\/ICC, ELISA applications with reactivity to human, rat samples\n25566-1-AP targets ALKBH8 in IHC, IF\/ICC, ELISA applications and shows reactivity with human, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive IHC detected in: rat kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: U2OS cells, HEK-293 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nALKBH8 (Alpha-ketoglutarate-dependent dioxygenase) is unique among the ALKBH family, since it harbors a 2-oxoglutarate and Fe(II)-dependent oxygenase domain as well as an RNA recognition motif and methyl transferase domain (PMID: 27329810). ALKBH8 is a \"writer\" enzyme that modifies the wobble uridine of tRNA and regulates protein translation, including that of selenoproteins (PMID: 38550277). ALKBH8 is highly expressed in high-grade, superficially and deeply invasive bladder cancer. ALKBH8 knockdown induces apoptosis in bladder cancer cells (PMID: 38550277).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag22239 Product name: Recombinant human ALKBH8 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 1-238 aa of BC015183 Sequence: MDSNHQSNYKLSKTEKKFLRKQIKAKHTLLRHEGIETVSYATQSLVVANGGLGNGVSRNQLLPVLEKCGLVDALLMPPNKPYSFARYRTTEESKRAYVTLNGKEVVDDLGQKITLYLNFVEKVQWKELRPQALPPGLMVVEEIISSEEEKMLLESVDWTEDTDNQNSQKSLKHRRVKHFGYEFHYENNNVDKDKPLSGGLPDICESFLEKWLRKGYIKHKPDQMTINQYEPGQDCHGF Predict reactive species\nFull Name: alkB, alkylation repair homolog 8 (E. coli)\nCalculated Molecular Weight: 664 aa, 75 kDa\nGenBank Accession Number: BC015183\nGene Symbol: ALKBH8\nGene ID (NCBI): 91801\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q96BT7\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869807235241,"sku":"25566-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-25566-1-ap","provider":"Iright","version":"1.0","type":"link"}