{"product_id":"proteintech-25731-1-ap","title":"Proteintech, 25731-1-AP, CERK Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe CERK (25731-1-AP) by Proteintech is a Polyclonal antibody targeting CERK in WB, IHC, ELISA applications with reactivity to human, mouse samples\n25731-1-AP targets CERK in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse heart tissue, mouse liver tissue\nPositive IHC detected in: mouse heart tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCeramide kinase (CERK) is a crucial lipid kinase that specifically catalyses ceramide (CER) to ceramide-1-phosphate. It plays a vital role in biological functions and certain diseases, including aberrant wound healing (PMID: 24823941), obesity-diabetes (PMID: 22465662), mesangio-proliferativekidney diseases (PMID: 25134723), cancer (PMID: 25164007) and neuroblastoma (PMID: 22579669). CERK has 2 isoforms with the molecular weight of 60 and 38 kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag22774 Product name: Recombinant human CERK protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 224-537 aa of BC126940 Sequence: AVLVPSSLRIGIIPAGSTDCVCYSTVGTSDAETSALHIVVGDSLAMDVSSVHHNSTLLRYSVSLLGYGFYGDIIKDSEKKRWLGLARYDFSGLKTFLSHHCYEGTVSFLPAQHTVGSPRDRKPCRAGCFVCRQSKQQLEEEQKKALYGLEAAEDVEEWQVVCGKFLAINATNMSCACRRSPRGLSPAAHLGDGSSDLILIRKCSRFNFLRFLIRHTNQQDQFDFTFVEVYRVKKFQFTSKHMEDEDSDLKEGGKKRFGHICSSHPSCCCTVSNSSWNCDGEVLHSPAIEVRVHCQLVRLFARGIEENPKPDSHS Predict reactive species\nFull Name: ceramide kinase\nCalculated Molecular Weight: 537 aa, 60 kDa, 38 kDa\nObserved Molecular Weight: 38 kDa\nGenBank Accession Number: BC126940\nGene Symbol: CERK\nGene ID (NCBI): 64781\nRRID: AB_2880214\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q8TCT0\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869524185257,"sku":"25731-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-25731-1-ap","provider":"Iright","version":"1.0","type":"link"}