{"product_id":"proteintech-27311-1-ap","title":"Proteintech, 27311-1-AP, SERCA2\/ATP2A2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe SERCA2\/ATP2A2 (27311-1-AP) by Proteintech is a Polyclonal antibody targeting SERCA2\/ATP2A2 in WB, IHC, IF\/ICC, IF-P, IF-Fro, ELISA applications with reactivity to human, mouse samples\n27311-1-AP targets SERCA2\/ATP2A2 in WB, IHC, IF\/ICC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse heart tissue, HEK-293 cells,  mouse heart unboiled,  37°C incubated HEK-293 cells\nPositive IHC detected in: mouse skeletal muscle tissue, mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: mouse heart tissue\nPositive IF-Fro detected in: mouse heart tissue\nPositive IF\/ICC detected in: C2C12 cells, mouse heart tissue\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)-P: IF-P : 1:50-1:500\nImmunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nATP2A2, also known as SERCA2 (Sarco\/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. ATP2A2 is mainly expressed in the sarcoplasmic reticulum of muscle cells and the endoplasmic reticulum of most cell types.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag26177 Product name: Recombinant human SERCA2,ATP2A2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 314-580 aa of BC035588 Sequence: VITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCRMFILDRVEGDTCSLNEFTITGSTYAPIGEVHKDDKPVNCHQYDGLVELATICALCNDSALDYNEAKGVYEKVGEATETALTCLVEKMNVFDTELKGLSKIERANACNSVIKQLMKKEFTLEFSRDRKSMSVYCTPNKPSRTSMSKMFVKGAPEGVIDRCTHIRVGSTKVPMTSGVKQKIMSVIREWGSGSDTLRCLALATHDNPLRREEMHLEDS Predict reactive species\nFull Name: ATPase, Ca++ transporting, cardiac muscle, slow twitch 2\nCalculated Molecular Weight: 1042 aa, 115 kDa\nObserved Molecular Weight: 110-115 kDa\nGenBank Accession Number: BC035588\nGene Symbol: SERCA2\nGene ID (NCBI): 488\nRRID: AB_3085945\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P16615\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868945109161,"sku":"27311-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-27311-1-ap","provider":"Iright","version":"1.0","type":"link"}