{"product_id":"proteintech-27406-1-ap","title":"Proteintech, 27406-1-AP, PD-L2\/CD273 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PD-L2\/CD273 (27406-1-AP) by Proteintech is a Polyclonal antibody targeting PD-L2\/CD273 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n27406-1-AP targets PD-L2\/CD273 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: NIH\/3T3 cells, Raji cells,  mouse heart tissue,  rat heart tissue\nPositive IHC detected in: mouse heart tissue, rat heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: NIH\/3T3 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:3000\nImmunohistochemistry (IHC): IHC : 1:1000-1:4000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nProgrammed cell death 1 ligand 2 (PDCD1LG2), also known as PD-L2, CD273 and B7-DC, is an immune checkpoint receptor ligand which plays a role in negative regulation of the adaptive immune response. PDCD1LG2\/PD-L2 protein is expressed mainly by dendritic cells, macrophages, and tumor cells, and down-regulates the effector functions of T cells via PD-1\/PD-L2 axis in the tumor microenvironment (PMID: 30275216). The endogenous PDCD1LG2\/PD-L2 protein displays a molecular weight of ∼45 kDa(glycosylated form), and a minor band at 35 kDa (non-glycosylated form) in western blot (PMID: 28811964, PMID: 34697216).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag26384 Product name: Recombinant human PDCD1LG2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 20-220 aa of BC074766 Sequence: LFTVTVPKELYIIEHGSNVTLECNFDTGSHVNLGAITASLQKVENDTSPHRERATLLEEQLPLGKASFHIPQVQVRDEGQYQCIIIYGVAWDYKYLTLKVKASYRKINTHILKVPETDEVELTCQATGYPLAEVSWPNVSVPANTSHSRTPEGLYQVTSVLRLKPPPGRNFSCVFWNTHVRELTLASIDLQSQMEPRTHPT Predict reactive species\nFull Name: programmed cell death 1 ligand 2\nCalculated Molecular Weight: 273 aa, 31 kDa\nObserved Molecular Weight: 45 kDa\nGenBank Accession Number: BC074766\nGene Symbol: PD-L2\nGene ID (NCBI): 80380\nRRID: AB_3085954\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9BQ51\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887755645097,"sku":"27406-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-27406-1-ap","provider":"Iright","version":"1.0","type":"link"}