{"product_id":"proteintech-27751-1-ap","title":"Proteintech, 27751-1-AP, APOB Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe APOB (27751-1-AP) by Proteintech is a Polyclonal antibody targeting APOB in WB, IHC, ELISA applications with reactivity to human, mouse samples\n27751-1-AP targets APOB in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse liver tissue, HepG2 cells\nPositive IHC detected in: mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe apolipoprotein B (APOB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The APOB encodes two different isoproteins through mRNA editing, APOB-48 and APOB-100. APOB-48 and APOB-100 is present in both human liver and intestine. APOB-100 is essential for the assembly of VLDL in the liver. APOB-48 is essential for the assembly of chylomicrons in the intestine. It is well established that APOB-100 levels are associated with coronary heart disease. Unlike humans and rabbits, rats and mice secrete both apoB-100- and apoB-48-containing lipoproteins from the liver as a result of the hepatic expression of APOBEC-1 (PMID: 9633945). This antibody recognizes both of APOB-48, APOB-100 (PMID:11839763, PMID:2450346).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag26773 Product name: Recombinant human APOB protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 23-220 aa of NM_000384 Sequence: MAGARAEEEMLENVSLVCPKDATRFKHLRKYTYNYEAESSSGVPGTADSRSATRINCKVELEVPQLCSFILKTSQCTLKEVYGFNPEGKALLKKTKNSEEFAAAMSRYELKLAIPEGKQVFLYPEKDEPTYILNIKRGIISALLVPPETEEAKQVLFLDTVYGNCSTHFTVKTRKGNVATEISTERDLGQCDRFKPIRT Predict reactive species\nFull Name: apolipoprotein B (including Ag(x) antigen)\nCalculated Molecular Weight: 516 kDa\nObserved Molecular Weight: 150-250 kDa, 400-520 kDa\nGenBank Accession Number: NM_000384\nGene Symbol: APOB\nGene ID (NCBI): 338\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P04114\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46884984357033,"sku":"27751-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-27751-1-ap","provider":"Iright","version":"1.0","type":"link"}