{"product_id":"proteintech-27808-1-ap","title":"Proteintech, 27808-1-AP, PARG Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PARG (27808-1-AP) by Proteintech is a Polyclonal antibody targeting PARG in WB, IHC, IP, ELISA applications with reactivity to human, mouse samples\n27808-1-AP targets PARG in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, Jurkat cells,  K-562 cells\nPositive IP detected in: Jurkat cells, mouse testis tissue\nPositive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPoly(ADP-ribose) glycohydrolase (PARG) is an enzyme that reverses the PARylation process by breaking down long chains of PAR polymers on proteins, thus having an opposite function to PARP. It hydrolyzes PAR into ADP-ribose units, terminating signals initiated by PARP enzymes. PARG's function is crucial for maintaining PAR balance within cells, which is essential for DNA repair, transcriptional regulation, and cell death. PARG possesses multiple isoforms with distinct subcellular localizations, and its activity is essential for cellular survival. (PMID: 38580335, PMID: 39546575)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, zebrafish\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag27137 Product name: Recombinant human PARG protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 183-441 aa of BC052966 Sequence: SNANIDRSPQNDDHSDTDSEENRDNQQFLTTVKLANAKQTTEDEQAREAKSHQKCSKSCDPGEDCASCQQDEIDVVPESPLSDVGSEDVGTGPKNDNKLTRQESCLGNSPPFEKESEPESPMDVDNSKNSCQDSEADEETSPGFDEQEDGSSSQTANKPSRFQARDADIEFRKRYSTKGGEVRLHFQFEGGESRTGMNDLNAKLPGNISSLNVECRNSKQHGKKDSKITDHFMRLPKAEDRRKEQWETKHQRTERKIPK Predict reactive species\nFull Name: poly (ADP-ribose) glycohydrolase\nCalculated Molecular Weight: 111 kDa\nObserved Molecular Weight: 100-111 kDa\nGenBank Accession Number: BC052966\nGene Symbol: PARG\nGene ID (NCBI): 8505\nRRID: AB_2880979\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q86W56\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868986036393,"sku":"27808-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-27808-1-ap","provider":"Iright","version":"1.0","type":"link"}