{"product_id":"proteintech-27874-1-ap","title":"Proteintech, 27874-1-AP, APBB1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe APBB1 (27874-1-AP) by Proteintech is a Polyclonal antibody targeting APBB1 in WB, IP, ELISA applications with reactivity to human, mouse, rat samples\n27874-1-AP targets APBB1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A549 cells, mouse brain tissue,  SH-SY5Y cells,  U-87 MG cells,  U2OS cells,  rat brain tissue\nPositive IP detected in: mouse brain tissue\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\n\u003cb\u003eBackground Information\u003c\/b\u003e\nAdaptor protein FE65 (APBB1) specifically binds to the intracellular tail of the type I transmembrane protein, β-amyloid precursor protein (APP). FE65 is a multimodular adaptor protein, consisting of three major protein-protein interaction domains, a WW domain and two phosphotyrosine interaction domains (PID). The interaction between FE65 and APP mainly takes place between the C-terminal PID (PID2) and the APP intracellular domain (AICD) (PMID: 15647266). There is novel evidence that the major isoform of FE65 (97-kDa FE65, p97FE65) can be converted to a 65-kDa N-terminally truncated C-terminal fragment (p65FE65) via endoproteolysis. The cleavage region locates immediately after an acidic residue cluster but before the three major protein-protein binding domains (PMID: 15647266). FE65 is predominantly expressed in central nervous system neurons, and its expression is regulated during development and aging, with high levels corresponding to the timing of neural tissue formation and high neuronal activity (PMID: 7867517, PMID: 10561691, PMID: 14689444). p65FE65 (65kDa) is endoproteolytically cleaved from p97FE65 (97 kDa), the major isoform of FE65 (97-kDa FE65, p97FE65) can be converted to a 65-kDa N-terminally truncated C-terminal fragment (p65FE65) via endoproteolysis (PMID: 15647266).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag27434 Product name: Recombinant human APBB1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 43-268 aa of BC010854 Sequence: TAVGPKDLRSAMGEGGGPEPGPANAKWLKEGQNQLRRAATAHRDQNRNVTLTLAEEASQEPEMAPLGPKGLIHLYSELELSAHNAANRGLRGPGLIISTQEQGPDEGEEKAAGEAEEEEEDDDDEEEEEDLSSPPGLPEPLESVEAPPRPQALTDGPREHSKSASLLFGMRNSAASDEDSSWATLSQGSPSYGSPEDTDSFWNPNAFETDSDLPAGWMRVQDTSGT Predict reactive species\nFull Name: amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65)\nCalculated Molecular Weight: 708 aa, 77 kDa\nObserved Molecular Weight: 65 kDa, 97 kDa\nGenBank Accession Number: BC010854\nGene Symbol: APBB1\nGene ID (NCBI): 322\nRRID: AB_3669628\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O00213\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46884971643049,"sku":"27874-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-27874-1-ap","provider":"Iright","version":"1.0","type":"link"}