{"product_id":"proteintech-28252-1-ap","title":"Proteintech, 28252-1-AP, Phospho-RIPK1 (Ser166) Polyclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-RIPK1 (Ser166) (28252-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-RIPK1 (Ser166) in WB, ELISA applications with reactivity to Human samples\n28252-1-AP targets Phospho-RIPK1 (Ser166) in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: TNF-alpha treated HT-29 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nRIPK1, a 74 kDa protein, is composed of a N-terminal kinase domain, an intermediate domain (containing the RIP homotypic interaction motif, RHIM) and a C-terminal death domain. Stimulation of cells with TNFα can promote distinct cell death pathways, including RIPK1-independent apoptosis, necroptosis, and RIPK1-dependent apoptosis (RDA). TNFα induces cell necroptosis and the phosphorylation of RIPK1 at the Ser166 residue i.e. p-RIPK1 (Ser166), both of which can be effectively inhibited by Nec-1. Therefore, p-RIPK1 (Ser166) is considered a biomarker for the activation of RIPK1 kinase and necroptosis (PMID: 31440386, PMID: 29891719).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: Human\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: receptor (TNFRSF)-interacting serine-threonine kinase 1\nCalculated Molecular Weight: 76 kDa\nObserved Molecular Weight: 70-80 kDa\nGenBank Accession Number: NM_003804\nGene Symbol: RIPK1\nGene ID (NCBI): 8737\nRRID: AB_2881098\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q13546\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868913684649,"sku":"28252-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-28252-1-ap","provider":"Iright","version":"1.0","type":"link"}