{"product_id":"proteintech-28733-1-ap","title":"Proteintech, 28733-1-AP, Phospho-ERK1\/2 (Thr202\/Tyr204) Polyclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-ERK1\/2 (Thr202\/Tyr204) (28733-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-ERK1\/2 (Thr202\/Tyr204) in WB, IP, ELISA applications with reactivity to human, mouse samples\n28733-1-AP targets Phospho-ERK1\/2 (Thr202\/Tyr204) in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: NIH\/3T3 cells, Calyculin A treated HEK-293T cells\nPositive IP detected in: Calyculin A treated PC-3 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSerine\/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1\/ERK2 and MAPK3\/ERK1 are the 2 MAPKs which play an important role in the MAPK\/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG\/SCF. Depending on the cellular context, the MAPK\/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK\/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202\/Tyr204 and Thr185\/Tyr187, respectively. Several downstream targets of p44\/42 have been identified, including p90RSK and the transcription factor Elk-1. This antibody simultaneously recognizes the phosphorylation of ERK1 at Thr202 and Tyr204, as well as the phosphorylation of ERK2 at Thr185 and Tyr187.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse, rat, pig, chicken, zebrafish, sheep, goat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: mitogen-activated protein kinase 3\nCalculated Molecular Weight: 38-43 kDa\nObserved Molecular Weight: 38-43 kDa\nGenBank Accession Number: NM_002746\nGene Symbol: ERK1\nGene ID (NCBI): 5595\nRRID: AB_2881202\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P27361\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868819181737,"sku":"28733-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-28733-1-ap","provider":"Iright","version":"1.0","type":"link"}