{"product_id":"proteintech-28755-1-ap","title":"Proteintech, 28755-1-AP, Phospho-FOXO3A (Ser315) Polyclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-FOXO3A (Ser315) (28755-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-FOXO3A (Ser315) in WB, ELISA applications with reactivity to human, mouse, rat samples\n28755-1-AP targets Phospho-FOXO3A (Ser315) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Calyculin A treated PC-3 cells, HeLa cells,  PC-3 cells,  TGF beta 1 treated HeLa cells,  Forskolin treated PC-3 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nIn mammalian cells, FoxO factors act as potent transcriptional activators that upregulate the expression of programs of genes involved in stress resistance, cell cycle arrest, differentiation, apoptosis, autophagy, and metabolism. FOXO3 has a key role in the maintenance of adult neural and hematopoietic stem cells. It also associates with extreme longevity in humans and regulates the homeostasis of adult stem cell pools in mammals, which may contribute to longevity. Phosphorylation inhibits FOXO3 nuclear translocation that is essential for its regulation and function. The calculated molecular weight of FOXO3 is 71 kDa,but some experiments analysis using a FOXO3a specific antibody showed the expression of a 90 kDa full length FOXO3a protein (Full) and a 40 kDa processed FOXO3a product (Short). 90 kDa FOXO3a protein is a phosphorylated via activation of PI3K (phosphatidylinositol 3-kinase) and Akt after INS stimulation, and translocate from the nucleus to the cytoplasm. (PMID: 19427386). IKBKE directly activates Akt and that Akt inhibits FOXO3a by phosphorylation of Ser32, Ser253 and Ser315.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, bovine\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: forkhead box O3\nCalculated Molecular Weight: 673 aa, 71 kDa\nObserved Molecular Weight: 80-90 kDa\nGenBank Accession Number: BC021224\nGene Symbol: FOXO3A\nGene ID (NCBI): 2309\nRRID: AB_2881210\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O43524\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868907720873,"sku":"28755-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-28755-1-ap","provider":"Iright","version":"1.0","type":"link"}