{"product_id":"proteintech-28792-1-ap","title":"Proteintech, 28792-1-AP, Phospho-CREB1 (Ser133) Polyclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-CREB1 (Ser133) (28792-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-CREB1 (Ser133) in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n28792-1-AP targets Phospho-CREB1 (Ser133) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Forskolin treated HSC-T6 cells, HeLa cells,  NIH\/3T3 cells,  Forskolin treated NIH\/3T3 cells\nPositive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:8000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe cAMP-response element binding protein (CREB) is localized in the nucleus and acts as a transcription factor, which binds to the cAMP response element (CRE) of the promoters of its target genes, upon phosphorylation at Ser133 by different receptor-activated protein kinases, such as protein kinase A (PKA), calmodulin-dependent protein kinase (CaMK), mitogen-activated protein kinases (MAPK), and other kinases. The activity of CREB1 in neurons has been correlated with various intracellular processes, including proliferation, differentiation, survival, long-term synaptic potentiation, neurogenesis, and neuronal plasticity. CREB1 belongs to the CREB\/activating transcription factor (ATF) family of transcription factors. (PMID: 30214393, PMID: 33917483, PMID: 32605164) The antibody also detects the phosphorylated form of the CREB-related protein ATF1.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: cAMP responsive element binding protein 1\nCalculated Molecular Weight: 341 aa, 35 kDa\nObserved Molecular Weight: 35-46 kDa\nGenBank Accession Number: BC010636\nGene Symbol: CREB1\nGene ID (NCBI): 1385\nRRID: AB_2918203\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P16220\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868828356777,"sku":"28792-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-28792-1-ap","provider":"Iright","version":"1.0","type":"link"}