{"product_id":"proteintech-28873-1-ap","title":"Proteintech, 28873-1-AP, Phospho-GRB10 (Ser476) Polyclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-GRB10 (Ser476) (28873-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-GRB10 (Ser476) in WB, ELISA applications with reactivity to Human, Mouse samples\n28873-1-AP targets Phospho-GRB10 (Ser476) in WB, ELISA applications and shows reactivity with Human, Mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: λ phosphatase treated NIH\/3T3 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nGrowth factor receptor-bound protein 10 (GRB10) is an adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. GRB10 has three consensus domains including pleckstrin homology (PH) domain, SH2\/SH3 domain and Ras-associating domain. By binding to kinases, GRB10 suppresses signals from activated receptors tyrosine kinases, including the INSR and INS-like growth factor (IGF1R) receptors. It may play a role in mediating INS-stimulated ubiquitination of INSR, leading to proteasomal degradation. It has been reported that GRB10 is a direct substrate of mechanistic\/mammalian target of mTOR and can be  phosphorylated at Ser150, Ser428 and Ser476 identified as MAPK-mediated phosphorylation sites. (PMID: 15952796, 24746805, 21659605)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: Human, Mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: growth factor receptor-bound protein 10\nCalculated Molecular Weight: 536aa,61 kDa; 594aa,67 kDa\nObserved Molecular Weight: 70-80 kDa\nGenBank Accession Number: BC024285\nGene Symbol: GRB10\nGene ID (NCBI): 2887\nRRID: AB_2918210\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q13322\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887759872169,"sku":"28873-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-28873-1-ap","provider":"Iright","version":"1.0","type":"link"}