{"product_id":"proteintech-28953-1-ap","title":"Proteintech, 28953-1-AP, Phospho-MST1 (Thr183)\/MST2 (Thr180) Polyclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-MST1 (Thr183)\/MST2 (Thr180) (28953-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-MST1 (Thr183)\/MST2 (Thr180) in WB, ELISA applications with reactivity to Human samples\n28953-1-AP targets Phospho-MST1 (Thr183)\/MST2 (Thr180) in WB, ELISA applications and shows reactivity with Human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Staurosporine treated Ramos cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMammalian STE20-like serine-threonine kinase MST1, encoded by the STK4 gene, is a multifunctional protein. MST1 and its closest paralogs MST2 (encoded by the STK3 gene), MST3, and MST4 are members of the Class II Germinal Center Family of Protein Kinases. MST1\/2 and LATS1\/2 (large tumor suppressor 1 and 2) are core kinase components of the Hippo tumor suppressor pathway in mammalians . In the conventional Hippo pathway, the MST1\/2 and LATS1\/2 signaling cascade phosphorylates and inactivates the transcriptional coactivator YAP1 (yes associated protein 1) and its close paralog WWTR1]. YAP1 and WWTR1 do not have DNA binding domains and they exert their biological outputs, such as cell proliferation and survival, by interacting with the TEAD1-4 transcription factors. Lines of evidence have indicated that dysregulation or loss of STK4\/Hippo signaling is linked to developmental disorders and carcinogenesis with poor prognosis. MST1 is a stress-induced kinase and it can be activated in response to cell-death inducers. Autophosphorylation of MST1 at Thr183 (Thr180 in MST2) in the activation loop is a key activation mechanism for MST1\/2 because phosphorylation of Thr183\/180 causes the cleavage of MST1 by caspases under apoptotic conditions.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: Human\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: serine\/threonine kinase 4\nCalculated Molecular Weight: 56 kDa\nObserved Molecular Weight: 52-56 kDa\nGenBank Accession Number: BC005231\nGene Symbol: MST1\nGene ID (NCBI): 6789\nRRID: AB_2918221\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q13043\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868959232169,"sku":"28953-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-28953-1-ap","provider":"Iright","version":"1.0","type":"link"}