{"product_id":"proteintech-29032-1-ap","title":"Proteintech, 29032-1-AP, Phospho-eEF2K (Ser366) Polyclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-eEF2K (Ser366) (29032-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-eEF2K (Ser366) in WB, ELISA applications with reactivity to Human samples\n29032-1-AP targets Phospho-eEF2K (Ser366) in WB, ELISA applications and shows reactivity with Human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Jurkat cells, λ phosphatase treated Jurkat cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nEukaryotic Elongation Factor-2 Kinase (eEF2K) acts as a negative regulator of protein synthesis, translation, and cell growth. As a structurally unique member of the alpha-kinase family, eEF2K is essential to cell survival under stressful conditions, as it contributes to both cell viability and proliferation. eEF2K is regulated by various mechanisms, including phosphorylation through residues and autophosphorylation. eEF2K is regulated by various mechanisms, including phosphorylation through residues and autophosphorylation. eEF2K is downregulated through the phosphorylation of multiple sites via mTOR signaling and upregulated via the AMPK pathway. In the S6K-mediated pathway, RPS6KB1 or p70S6K, can phosphorylate eEF2K on Ser-366, rendering it inactive(PMID: 11500364, PMID: 34532346). The calculated molecular weight of eEF2K is 82kDa, and probably due to phosphorylation modification, resulting in a larger molecular weight, around 100kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: Human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: eukaryotic elongation factor-2 kinase\nCalculated Molecular Weight: 725 aa, 100 kDa\nObserved Molecular Weight: 100 kDa\nGenBank Accession Number: BC032665\nGene Symbol: EEF2K\nGene ID (NCBI): 29904\nRRID: AB_2918231\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O00418\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887759577257,"sku":"29032-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-29032-1-ap","provider":"Iright","version":"1.0","type":"link"}