{"product_id":"proteintech-29935-1-ap","title":"Proteintech, 29935-1-AP, EIF2B5 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe EIF2B5 (29935-1-AP) by Proteintech is a Polyclonal antibody targeting EIF2B5 in WB, IHC, IF\/ICC, ELISA applications with reactivity to Human,  mouse,  rat samples\n29935-1-AP targets EIF2B5 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with Human,  mouse,  rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A549 cells, HEK-293T cells,  HT-29 cells,  HeLa cells,  Jurkat cells,  NIH\/3T3 cells,  mouse brain tissue,  rat brain tissue\nPositive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunohistochemistry (IHC): IHC : 1:200-1:800\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nEIF2B5 encodes translation initiation factor eIF-2B subunit epsilon protein (PMID 8688466). EIF2B5 mutations compromise the induction of GFAP-expressing astrocytes in VWM (vanishing white matter) leukodystrophy (PMID:15723074, PMID:15776425). The upregulation of EIF2B5 is associated with cancer such as human pulmonary adenocarcinoma cell and breast cancer (PMID:24366584, PMID:24979338). EIF2B5 also has 65kDa truncated protein isoform (PMID:28961236).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: Human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag32175 Product name: Recombinant human EIF2B5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 455-711 aa of NM_003907.2 Sequence: DAEEDEDDGEFSDDSGADQEKDKVKMKGYNPAEVGAAGKGYLWKAAGMNMEEEEELQQNLWGLKINMEEESESESEQSMDSEEPDSRGGSPQMDDIKVFQNEVLGTLQRGKEENISCDNLVLEINSLKYAYNISLKEVMQVLSHVVLEFPLQQMDSPLDSSRYCALLLPLLKAWSPVFRNYIKRAADHLEALAAIEDFFLEHEALGISMAKVLMAFYQLEILAEETILSWFSQRDTTDKGQQLRKNQQLQRFIQWLK Predict reactive species\nFull Name: eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa\nCalculated Molecular Weight: 80 kDa\nObserved Molecular Weight: 65-80 kDa\nGenBank Accession Number: NM_003907.2\nGene Symbol: EIF2B5\nGene ID (NCBI): 8893\nRRID: AB_2923623\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q13144\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869678293161,"sku":"29935-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-29935-1-ap","provider":"Iright","version":"1.0","type":"link"}