{"product_id":"proteintech-30139-1-ap","title":"Proteintech, 30139-1-AP, EGFR Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe EGFR (30139-1-AP) by Proteintech is a Polyclonal antibody targeting EGFR in WB, IF\/ICC, FC, IP, ELISA applications with reactivity to human samples\n30139-1-AP targets EGFR in WB, IF\/ICC, FC, IP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A431 cells\nPositive IP detected in: HeLa cells\nPositive IF\/ICC detected in: A431 cells, HaCaT cells\nPositive FC detected in: A431 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:750-1:3000\nFlow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nEGFR is a receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. ERBB1 is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1\/beta-catenin.  Isoform 2\/truncated isoform of ERBB1 may act as an antagonist. EGFR is associated with lung cancer.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Recombinant protein Predict reactive species\nFull Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)\nCalculated Molecular Weight: 134kd\nObserved Molecular Weight: 145-165 kDa\nGenBank Accession Number: NM_005228.5\nGene Symbol: EGFR\nGene ID (NCBI): 1956\nRRID: AB_3086243\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P00533\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869678227625,"sku":"30139-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-30139-1-ap","provider":"Iright","version":"1.0","type":"link"}