{"product_id":"proteintech-30376-1-ap","title":"Proteintech, 30376-1-AP, Phospho-RB1 (Ser807\/811) Polyclonal antibody","description":"Size: 20ul \/ 100ul\nPhospho-RB1 (Ser807\/811) Polyclonal Antibody for WB, ELISA\n30376-1-AP targets Phospho-RB1 (Ser807\/811) in WB, ELISA applications and shows reactivity with Human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Calyculin A treated Jurkat cells, Jurkat cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nRB1, also named as pp110, pRb and p105 Rb, belongs to the retinoblastoma protein (RB) family. Phosphorylation and dephosphorylation of RB1 is well known to be a key regulator in cell cycle progression in cancer cells. Phosphorylation of RB1 at multiple S\/T residues, first by cyclin D-dependent kinases and subsequently by cyclin E-CDK2 in later G1 phase, completely inactivates RB1, enabling the release of E2F from RB1 complexes and activation of E2F-mediated transcription. Monomethylation at Lys810 by SMYD2 enhances phosphorylation at Ser807 and Ser811, and promotes cell cycle progression. (PMID: 22787429, PMID: 21115810, PMID: 32344731)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: Human\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: retinoblastoma 1\nCalculated Molecular Weight: 928 aa, 106 kDa\nObserved Molecular Weight: 110 kDa\nGenBank Accession Number: BC039060\nGene Symbol: RB1\nGene ID (NCBI): 5925\nRRID: AB_3085351\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P06400\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868959330473,"sku":"30376-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-30376-1-ap","provider":"Iright","version":"1.0","type":"link"}