{"product_id":"proteintech-31133-1-ap","title":"Proteintech, 31133-1-AP, Phospho-INSR (Tyr1150\/1151)\/IGF1R (Tyr1135\/1136) Polyclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-INSR (Tyr1150\/1151)\/IGF1R (Tyr1135\/1136) (31133-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-INSR (Tyr1150\/1151)\/IGF1R (Tyr1135\/1136) in WB, ELISA applications with reactivity to human samples\n31133-1-AP targets Phospho-INSR (Tyr1150\/1151)\/IGF1R (Tyr1135\/1136) in WB, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: IGF-1 treated HEK-293T cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nInsulin binding to the insulin receptor (INSR) triggers sequential conformational changes and autophosphorylation of the receptor, followed by activation of a kinase signaling cascade that plays essential roles in a wide variety of biological processes. INSR belongs to a class of receptor tyrosine kinases (RTKs) that comprises 58 receptors in humans. The INSR shares a high structural homology with the IGF1R (84% similarity in the tyrosine kinase domain, 45-65% in the ligand-binding domain, and more than 50% in the overall amino acid sequence). In addition, ligand-dependent activation of the INSR and IGF1R activates almost identical downstream signaling cascades. Insulin binds to INSR in peripheral tissues, initiating receptor activation followed by intracellular signaling cascades. The first step in INSR activation is the autophosphorylation of intracellular tyrosine residues in the JM domain, kinase activation loop, and CT domain. Phosphorylation of three tyrosine residues (Tyr1146, Tyr1150, and Tyr1151, based on INSR isoform A numbering) located in the kinase activation loop plays a crucial role in kinase activity regulation. Insulin binding also induces INSR kinase-mediated phosphorylation of four tyrosine residues located in the JM (Tyr953 and Tyr960) and CT domain (Tyr1316 and Tyr1322). (PMID: 37779149,PMID:24434591\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: INSR\nObserved Molecular Weight: 95 kDa\nGenBank Accession Number: BC117172\nGene Symbol: INSR\nGene ID (NCBI): 3643\nRRID: AB_3669868\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P06213\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869728985257,"sku":"31133-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-31133-1-ap","provider":"Iright","version":"1.0","type":"link"}