{"product_id":"proteintech-31234-1-ap","title":"Proteintech, 31234-1-AP, RC3H1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe RC3H1 (31234-1-AP) by Proteintech is a Polyclonal antibody targeting RC3H1 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse samples\n31234-1-AP targets RC3H1 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: 4T1 cells, HEK-293T cells,  HeLa cells\nPositive IHC detected in: human ovary cancer tissue, mouse pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nRoquin-1 (Rc3h1) is an E3 ubiquitin ligase originally discovered in a mutational screen for genetic factors contributory to systemic lupus erythematosus-like symptoms in mice. A single base pair mutation in the Rc3h1 gene resulted in the manifestation of autoantibody production and sustained immunological inflammation characterized by excessive T follicular helper cell activation and formation of germinal centers. (PMID: 26673963)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag35089 Product name: Recombinant human RC3H1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1000-1133 aa of NM_001300850 Sequence: NTLAGQSQPPPPPPPKWPGMISSEQLSLELHQVEREIGKRTRELSMENQCSLDMKSKLNTSKQAENGQPEPQNKVPAEDLTLTFSDVPNGSALTQENISLLSNKTSSLNLSEDPEGGGDNNDSQRSGVTPSSAP Predict reactive species\nFull Name: ring finger and CCCH-type zinc finger domains 1\nCalculated Molecular Weight: 126 kDa\nObserved Molecular Weight: 130-150 kDa\nGenBank Accession Number: NM_001300850\nGene Symbol: RC3H1\nGene ID (NCBI): 149041\nRRID: AB_3669912\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity Purification\nUNIPROT ID: Q5TC82\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887781105833,"sku":"31234-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-31234-1-ap","provider":"Iright","version":"1.0","type":"link"}