{"product_id":"proteintech-32026-1-ap","title":"Proteintech, 32026-1-AP, HECW2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe HECW2 (32026-1-AP) by Proteintech is a Polyclonal antibody targeting HECW2 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human samples\n32026-1-AP targets HECW2 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: MDA-MB-468 cells, A549 cells,  U-118 MG cells\nPositive IHC detected in: mouse cerebellum tissue, rat cerebellum tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: A549 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nHECW2, also known as NEDL2 (Nedd4-like E3 ubiquitin-protein ligase 2), is a member of the HECT (Homologous to the E6-AP Carboxyl Terminus) family of E3 ubiquitin ligases. It is crucial in various pathophysiological processes, including cell proliferation, apoptosis, autophagy, immune response, and DNA damage repair (PMID: 39529070). HECW2 is known to stabilize and enhance the transcriptional activity of p73, a member of the p53 family of tumor suppressors, which promotes cell cycle arrest and apoptosis and has an essential role in neurodevelopment (PMID: 27389779). In cancer, HECW2 is a promising target for tumor therapy due to its role in regulating cell cycle progression and angiogenesis (PMID: 39529070). In neurodevelopmental disorders, variants in HECW2 have been reported to cause a disorder characterized by hypotonia, seizures, and impaired language (PMID: 34321324).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag36316 Product name: Recombinant human HECW2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 450-650 aa of XM_017004563.1 Sequence: SFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRADDGSLTSQTKLEDNPVENEEASTHEAASFEDKPENLPELAESSLPAGPAPEEGEGGPEPQPSADQGSAELCGSQEVDQPTSGADTGTSDASGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLECADSSCNESVT Predict reactive species\nFull Name: HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2\nCalculated Molecular Weight: 176 kDa，1572aa\nObserved Molecular Weight: 240 kDa\nGenBank Accession Number: XM_017004563.1\nGene Symbol: HECW2\nGene ID (NCBI): 57520\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity Purification\nUNIPROT ID: Q9P2P5\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887666319529,"sku":"32026-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-32026-1-ap","provider":"Iright","version":"1.0","type":"link"}