{"product_id":"proteintech-32217-1-ap","title":"Proteintech, 32217-1-AP, GCC2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe GCC2 (32217-1-AP) by Proteintech is a Polyclonal antibody targeting GCC2 in WB, IHC, ELISA applications with reactivity to human samples\n32217-1-AP targets GCC2 in WB, IHC, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293T cells, HeLa cells,  Jurkat cells,  Raji cells\nPositive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:2000-1:8000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe GCC2 protein, fully named GRIP and Coiled-Coil Domain-Containing Protein 2, is a key adaptor protein in the intracellular Golgi-endosomal membrane transport system. It specifically anchors to the Golgi membrane via its GRIP domain and is primarily responsible for mediating retrograde vesicular transport from endosomes to the Golgi apparatus, participating in the recycling and sorting of various cargo proteins, such as the mannose-6-phosphate receptor. Recent studies have revealed that GCC2 regulates the subcellular localization of the GATOR complex, thereby acting as an important upstream regulator of the mTORC1 signaling pathway, which influences cell growth, metabolism, and autophagy. Dysfunction of GCC2 is closely associated with neurodevelopmental disorders and tumorigenesis, underscoring its significance in cell biology and disease research.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag17535 Product name: Recombinant human GCC2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-343 aa of BC146789 Sequence: MKQEVEDSVTKMGDAHKELEQSHINYVKEIENLKNELMAVRSKYSEDKANLQKQLEEAMNTQLELSEQLKFQNNSEDNVKKLQEEIEKIRPGFEEQILYLQKQLDATTDEKKETVTQLQNIIEANSQHYQKNINSLQEELLQLKAIHQEEVKELMCQIEASAKEHEAEINKLNELKENLVKQCEASEKNIQKKYECELENLRKATSNANQDNQICSILLQENTFVEQVVNEKVKHLEDTLKELESQHSILKDEVTYMNNLKLKLEMDAQHIKDEFFHEREDLEFKINELLLAKEEQGCVIEKLKSELAGLNKQFCYTVEQHNREVQSLKEQHQKEISELNETF Predict reactive species\nFull Name: GRIP and coiled-coil domain containing 2\nCalculated Molecular Weight: 1583 aa, 185 kDa\nObserved Molecular Weight: 200 kDa\nGenBank Accession Number: BC146789\nGene Symbol: GCC2\nGene ID (NCBI): 9648\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity Purification\nUNIPROT ID: Q8IWJ2\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887648526505,"sku":"32217-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-32217-1-ap","provider":"Iright","version":"1.0","type":"link"}