{"product_id":"proteintech-32534-1-ap","title":"Proteintech, 32534-1-AP, Siglec-12 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe Siglec-12 (32534-1-AP) by Proteintech is a Polyclonal antibody targeting Siglec-12 in WB, IHC, ELISA applications with reactivity to mouse, rat samples\n32534-1-AP targets Siglec-12 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse spleen tissue, rat spleen tissue\nPositive IHC detected in: mouse spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily that contain sialoside-binding N-terminal domains (PMID: 22749793). Sialic acid binding Ig-like lectin 12 (Siglec-12, also known as S2V, SLG, SIGLECL1, and Siglec-XII) is a member of the SIGLEC3-like subfamily of SIGLECs, which is highly expressed in the spleen, such as CD8+ cells, macrophages, neutrophils, and dendritic cells. It mediates sialic-acid-dependent binding to cells and could be an identification of therapeutic targets and prognostic biomarkers of sarcomas (PMID: 38182638).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Eg2733 Product name: Recombinant Mouse Siglec-12 protein (rFc Tag) Source: mammalian cells -derived, V37 Tag: C-rFc Domain: 19-350 aa of NM_031181.2 Sequence: QNPQEVFTLNVERKVVVQEGLCVLVPCNFSYLKKRLTDWTDSDPVHGFWYREGTDRRKDSIVATNNPIRKAVKETRNRFFLLGDPWRNDCSLNIREIRKKDAGLYFFRLERGKTKYNYMWDKMTLVVTALTNTPQILLPETLEAGHPSNLTCSVPWDCGWTAPPIFSWTGTSVSFLSTNTTGSSVLTITPQPQDHGTNLTCQVTLPGTNVSTRMTIRLNVSYAPKNLTVTIYQGADSVSTILKNGSSLPISEGQSLRLICSTDSYPPANLSWSWDNLTLCPSKLSKPGLLELFPVHLKHGGVYTCQAQHALGSQHISLSLSPQSSATLSEMM Predict reactive species\nFull Name: sialic acid binding Ig-like lectin E\nCalculated Molecular Weight: 52 kDa\nObserved Molecular Weight: 70 kDa\nGenBank Accession Number: NM_031181.2\nGene Symbol: Siglec-E\nGene ID (NCBI): 83382\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity Purification\nUNIPROT ID: Q91Y57\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887801847977,"sku":"32534-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-32534-1-ap","provider":"Iright","version":"1.0","type":"link"}